Protocols

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Phylogenomics protocol from AGRF 
1. Oct 2022 protocol

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Reference genomes protocols – DNA extraction (long read)
1. Acacia pycnantha DNA extraction
2. Telopea speciosissima DNA extraction
3. Hibbertia scandens DNA extraction
4. Thelymitra variegata DNA extraction

5. Leucochrysum albicans, Phebalium stellatum, Wahlenbergia ceracea, Xanthorrhoea johnsonii DNA extraction
a. HMW DNA preparation
b. Optional sorbitol wash in 50 mL falcons (recommended)
c. Additional reading – High-molecular weight DNA extraction, clean-up and size selection for long-read sequencing

Reference genomes protocols – Whole-genome sequencing (WGS)
1. Phebalium stellatum and Xanthorrhoea johnsonii WGS

Reference genomes protocols – RNA extraction
1. Acacia pycnantha RNA extraction

Reference genomes protocols – Hi-C preparation
1. Phebalium stellatum, Wahlenbergia ceracea and Xanthorrhoea johnsonii Hi-C preparation
a. Phase Genomics Proximo Hi-C (Plant) Kit KT3040 Protocol Version 4.0 (February 2021)

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Project Agreements and Policies: Documents here
Data Access and Data Policy: More information here
Acknowledging the consortium: Wording here
Project information: here
Useful plant resources: here
Media: here

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